FIG. 7.

Notch signaling represses CD4 transcription element function and endogenous gene expression. (A) Transfection of the pT-based vectors into the D10 T_H clone. Transfection of the CMV-NotchIC expression plasmid (N) and of the empty CMV expression plasmid (P) is indicated. Data are presented and normalized as described for the data in Fig. 6. Multiple transfection experiments were conducted; presented are averages of data from three independent transfections. (B) Representative two-color flow cytometric plot of D10 cells cotransfected with the CMV-GFP and CMV-NotchIC expression plasmids. Transfections were conducted and analyzed as described in the legend to Fig. 6. (C) Percentage of transfected CD4^dull SP D10 T_H cells plotted against the amount of CMV-NotchIC plasmid added to the transfection. Multiple experiments were conducted; shown are two representative experiments.