FIG. 10.

AML-1/ETO-mediated repression cosegregates with the ability to interact with the central domain of N-CoR. The rat NP-3 promoter was activated approximately 50- to 60-fold by a combination of AML-1B and C/EBPα and repression by AML-1/ETO, and the indicated AML-1/ETO mutants were assessed. (A) Mapping of the domains required for transcriptional repression. The mutant ETOs used in Fig. 1 were transferred into AML-1/ETO, and the ability of these mutant ETOs to repress AML-1B-C/EBPα-dependent activation was measured and is expressed as fold repression. (B) Both predicted ZF motifs are required for AML-1/ETO function. Small deletions and single amino acid changes in the MYND domain of ETO (depicted as GAL4-ETO mutations in Fig. 2A) were transferred into AML-1/ETO and tested for repression of NP-3-activated transcription. The bars indicate average results of duplicate (A) or triplicate (B) experiments. WT, wild type.