FIG. 11.
An HDAC inhibitor impairs AML-1/ETO-mediated repression of the p21Waf1/Cip1 promoter. (A) Schematic diagram of the p21Waf1/Cip1 promoter. Open boxes represent perfect matches for the AML-1 consensus binding site, and dark boxes represent 5-of-6-bp matches for the consensus binding site. (B) TSA blocks AML-1/ETO-mediated repression. Repression assays were performed by using 2 μg of AML-1/ETO in the absence or presence of 300 nM TSA. The control levels were arbitrarily set to 1. A Rous sarcoma virus LTR-renilla luciferase plasmid was used as an internal control. The bars represent average results of duplicate experiments. A/E, AML-1/ETO.