FIG. 7.

Endogenous ETO cosediments with mSin3A and N-CoR. HEL cell lysates (2 mg of total protein) were fractionated on a 10 to 30% sucrose gradient. A 40-μl sample of each fraction was separated on an 8% polyacrylamide gel and then subjected to sequential immunoblot analysis using ETO, mSin3A, and HDAC-1 antibodies. The upper portion of the blot was independently probed with N-CoR antibodies. The standards are as follows: 669 kDa, thyroglobulin; 464 kDa, a tetramer of β-galactosidase; 292 kDa, a trimer of phosphorylase b; 200 kDa, myosin. The values on the right are molecular sizes in kilodaltons.