FIG. 9.

ETO and AML-ETO are associated with HDAC activity. (A) L293 cells were transfected with an HA-tagged ETO expression plasmid, and cell lysates were immunoprecipitated with anti-HA antibodies or with normal rabbit serum (NRS). (B) Cos-7 or L293 (C) cells were transfected with Flag-tagged AML-1/ETO (A/E) or MAD expression plasmids and immunoprecipitated with anti-Flag or anti-MAD antibodies or with normal rabbit serum. Untransfected Cos-7 or L293 cells were also immunoprecipitated with anti-Flag or anti-HA antibodies. HDAC activity was assayed in the immune complexes. Con, control; dpm, disintegrations per minute.