Genes	Stage 1	Stage 2	Stage 3	Stage 4	Stage 5
ATXN1	95 ℃, 5 min	95 ℃, 30 s; 67 ℃, 30 s; 72 ℃, 30 s; 25 cycles	72 ℃, 10 min	4 ℃, hold
ATXN2	95 ℃, 5 min	95 ℃, 45 s; 66 ℃(-0.5 ℃/cycle), 30 s; 72 ℃, 1 min; 10 cycles	95 ℃, 45 s; 62 ℃, 30 s; 72 ℃, 1 min; 35 cycles	72 ℃, 10 min	4 ℃, hold
ATXN3	95 ℃, 5 min	95 ℃, 30 s; 62 ℃(-0.5 ℃/cycle), 30 s; 72 ℃, 30 s; 10 cycles	95 ℃, 30 s; 52 ℃, 30 s; 72 ℃, 30 s; 35 cycles	72 ℃, 10 min	4 ℃, hold
CACNA1A	95 ℃, 5 min	95 ℃, 45 s; 62 ℃(-1 ℃/cycle), 50 s; 72 ℃, 1 min; 10 cycles	95 ℃, 45 s; 52 ℃, 50 s; 72 ℃, 1 min; 35 cycles	72 ℃, 10 min	4 ℃, hold
ATXN7	95 ℃, 5 min	95 ℃, 45 s; 70 ℃(-1 ℃/cycle), 50 s; 72 ℃, 1 min; 15 cycles	95 ℃, 45 s; 55 ℃, 50 s; 72 ℃, 1 min; 35 cycles	72 ℃, 10 min	4 ℃, hold
TBP	95 ℃, 5 min	95 ℃, 30 s; 65 ℃, 30 s; 72 ℃, 30 s; 40 cycles	72 ℃, 10 min	4 ℃, hold
ATN1	95 ℃, 5 min	95 ℃, 1 min; 68 ℃, 30 s; 72 ℃, 1 min; 40 cycles	72 ℃, 10 min	4 ℃, hold
BAI1	95 ℃, 5 min	95 ℃, 30 s; 68 ℃, 30 s; 72 ℃, 30 s; 40 cycles	72 ℃, 10 min	4 ℃, hold
IT15	95 ℃, 5 min	95 ℃, 45 s; 66 ℃(-0.5 ℃/cycle), 30 s; 72 ℃, 1 min; 10 cycles	95 ℃, 45 s; 62 ℃, 30 s; 72 ℃, 1 min; 35 cycles	72 ℃, 10 min	4 ℃, hold
HDL2	95 ℃, 5 min	95 ℃, 45 s; 70 ℃(-1 ℃/cycle), 50 s; 72 ℃, 1 min; 15 cycles	95 ℃, 45 s; 55 ℃, 50 s; 72 ℃, 1 min; 35 cycles	72 ℃, 10 min	4 ℃, hold
KCNN3	95 ℃, 5 min	95 ℃, 30 s; 70 ℃, 30 s; 72 ℃, 30 s; 40 cycles	72 ℃, 10 min	4 ℃, hold