FIG. 3.

In vitro and in vivo interactions of AML1/ETO and AML1/ETOΔC with CoRs and HDAC. (a) Pulldown experiments. In vitro translated AML1/ETO or AML1/ETOΔC was precipitated with GST or A GST-SMRT RD fusion protein. (b) Coimmunoprecipitation of AML1/ETO or AML1/ETOΔC and N-CoR. Immunoprecipitates were obtained with an anti-Myc antibody or an appropriate control monoclonal antibody from 293T cells transfected with a Myc-tagged AML1/ETO or AML1/ETOΔC expression vector and blotted with anti-N-CoR or anti-Myc antibodies, as indicated. (c) HDAC activity of the indicated immunoprecipitates from 293T cells transfected with the indicated expression vectors. dpm; disintegrations per minute.