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. 2024 Mar 11;46(1):2327495. doi: 10.1080/0886022X.2024.2327495

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© 2024 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The terms on which this article has been published allow the posting of the Accepted Manuscript in a repository by the author(s) or with their consent.

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Figure 5. — Quercetin alleviated aberrant ferroptosis activation in HK-2 cells. (A) Western blot analyses of GPX4, xCT, ACSL4, and Nrf2 protein levels in each group of HK-2 cells. (B–E) Semi-quantitative assessments of GPX4, xCT, ACSL4, and Nrf2 from immunoblots. (F–I) Quantitative PCR results showing relative mRNA expressions of GPX4, xCT, ACSL4, and Nrf2 in the cells from each group. CON: control HK-2 cells; CON + QCT: control HK-2 cells receiving quercetin treatment; HG: HK-2 cells under HG incubation; HG + QCT: HK-2 cells under HG incubation receiving quercetin treatment; HG + Fer-1: HK-2 cell under HG incubation receiving Fer-1 treatment. Data are mean ± SD. Significance levels are indicated as follows: ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05, determined by one-way ANOVA.