Figure 2. 8,9-EET inhibited the erastin-induced ferroptosis in PANC-1 cells.

PANC-1 cells were treated by erastin (10 μmol/L) for 12 h with/without 8,9-EET (1 μmol/L) pretreatment for 30 min. A: Cell viability of PANC-1 cells was detected by CCK-8. B: Concentration of Fe²⁺ in PANC-1 cells was detected by a corresponding kit. C: LDH activity in the supernatant of PANC-1 cells was detected. D: MDA content in PANC-1 cells was detected. E-F: Expression of ACSL4 protein was detected with Western blotting. F-I: Gene expressions of ACSL4, FPN, and FSP1 were detected with real-time PCR. Data are presented as the mean±standard deviation, n=3, *P<0.05, **P<0.01, and ***P<0.001.