Figure 3. CYP2J2 knockdown exacerbated the erastin-induced ferroptosis in PANC-1 cells.

PANC-1 cells were infected with CYP2J2 shRNA or control shRNA mediated by lentivirus vector. A: Three days later, the CYP2J2 gene expression was detected with real-time PCR. B-C: Protein level of CYP2J2 was evaluated with Western blotting. D: Concentration of 8,9-DHET in PANC-1 cells was detected by ELISA. Then PANC-1 cells were treated with erastin (10 μmol/L) for 12 h. E: Cell viability was detected with CCK-8. F: Concentration of Fe²⁺ in PANC-1 cells was detected. G: MDA content in PANC-1 cells was detected. H: Gene expression of ACSL4 was detected with real-time PCR. I-J: Expression of ACSL4 protein was detected with Western blotting. K-L: Gene expression of FPN and FSP1 was detected by real-time PCR. Data are presented as the mean ± standard deviation, n=3, *P<0.05, **P<0.01, and ***P<0.001.