Figure 4. 8,9-EET partly restored the GPX4 expression in a PPARγ-dependent manner.

PANC-1 cells were treated with erastin with/without 8,9-EET pretreatment. A: Gene expression of GPX4 was detected with real-time PCR. B-C: GPX4 protein expression was detected with Western blotting. D: Concentration of Fe²⁺ in PANC-1 cells was detected. E: LDH activity in the supernatant of PANC-1 cells was detected. F: MDA content in PANC-1 cells was detected. G-H: GPX4 protein expression was detected with Western blotting and the effects of GW9662 on 8,9-EET's regulation in erastin-treated PANC-1 cells were investigated. Data are presented as the mean ± standard deviation, n=3, *P<0.05, **P<0.01, and ***P<0.001.