图3.

流式细胞术检测小鼠体内淋巴细胞亚群分布特征

Figure 3 Distribution of lymphocyte subsets in mice detected by flow cytometry

A: T cell tubes were stained with CD3-APC-cy7, CD4-BV510, CD8-FITC, CD62L-PE, and CD44-BV421 monoclonal antibodies. B cell tubes were stained with CD19-PE-percp-cy5.5, CD45R-PEcyTM7, and CD138-APC-MAB monoclonal antibodies. CD3⁺CD4⁺CD8^-CD62L^- subpopulations increase in all 3 groups except PBS. Rv2654 protein combined with BCG group is particularly obvious. Rv2654 protein combined with BCG can significantly stimulate CD4⁺T and CD8⁺T cells to differentiate into effector and memory T cells. B: There is a significant increase in the number of B cells in the Rv2654 group, the BCG group, and the Rv2654+BCG group (all CD19⁺ cells).