RPA1 domain C binds ssDNA specifically. Extracts of E. coli containing approximately 0.3 μg of recombinant protein were assayed in the presence of the indicated competitor, namely, 0.5 or 3 μg of unlabled lambda DNA before (double stranded [DS]) or after (single stranded [SS]) boiling or 3 μg of yeast tRNA (RNA). V, extract of E. coli expressing the vector alone or competed with 3 μg of ssDNA. A*, B*, and C*, RPA1 domains A, B, and C.