FIG. 3.

Amino acid sequence alignment of the four ssDNA binding domains of RPA. The amino acid sequences of the indicated proteins were aligned with the PILEUP program and optimized as described in Materials and Methods. The secondary structure of hsRPA1_181–422, presented at the top of the sequences, was taken from Bochkarev et al. (5). Highly conserved residues are indicated in white type on a black background, and the putative zinc-binding domain in domain C is shaded. Numbers to the right of the protein designations refer to amino acid numbers. Note that 14 amino acids are deleted from the scRPA2 sequence at residue 115. Hs, H. sapiens; Xl, Xenopus laevis; Sc, S. cerevisiae; Sp, Schizosaccharomyces pombe; Os, Oryza sativa; Cf, Crithidia fasiculata; Ce, Caenorhabditis elegans.