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Schematic representation of the generation of DC vaccines. Monocytes isolated from peripheral blood can be differentiated into DCs after culture with GM-CSF and IL-4. Moreover, there are two main types of DCs in peripheral blood: pDCs (plasmacytoid)and mDCs (myeloid). DCs must be loaded with peptides from tumor antigens, RNA-encoding tumor antigens or tumor lysates to obtain the final product: mature and pulsed DCs.
