FIG. 2.

Localization of the negative cis-regulatory element in the first intronic region of the α-MHC gene. (A) Expression of different 3′ deletion mutants of the α-MHC gene in primary cultures of cardiac myocytes. Bars represent the mean value of seven different experiments. (B) Diagram of the α-MHC gene sequences from bp −340 to +117. The regulatory sequences are shown within the labeled box. The positions of the first and second exons are illustrated by black boxes. (C) Sequences of the first exon of the α-MHC gene, which are slightly different from previously published sequences (30).