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. 2024 Feb 26;13(5):401. doi: 10.3390/cells13050401

Figure 4.

Figure 4

Obesity induces increased proliferation of CCR2+ monocytes/macrophages in skin wounds. Male mice were administered 2 mg BrdU via i.v. injection on day 3 and day 6 post-wounding. At 4 h post-BrdU injection, proliferation was evaluated. (A) Gating strategy for assessing proliferation of major macrophage/monocyte populations in skin, bone marrow, and peripheral blood. (B) Numbers of Ly6C+F4/80lo/- monocytes/macrophages and Ly6C-F4/80+ macrophages in S/G2/M phases of cell cycle (BrdU+FxCycle+) in wounds on days 3 and 6 post-injury in ND (black bar) and HFD (open bar) mice. (C,D) Numbers of CD115+Ly6C+ and CD115+Ly6C-monocytes in S phase of cell cycle (BrdU+) in peripheral blood and bone marrow, respectively. n = 4/group; Data are mean ± SEM; * p < 0.05 vs. ND group by ANOVA.