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. 2024 Feb 20;13(5):366. doi: 10.3390/cells13050366

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Migration of HuCC-T1 and Oz cells induced by hSC-conditioned medium. Human cell lines derived from iCCA tumors were seeded in the upper chamber of a transwell. Conditioned medium obtained from hSC primary cultures was placed in the lower chamber and migration assessed for HuCCT-T1 cells (panel A) and for Oz cells (panel B). As shown by the micrographs and related diagrams, hSC-conditioned medium increased the migration of iCCA cells through the insert. Student’s t-test * p ≤ 0.05; ** p ≤ 0.01. N = 3.