FIG. 6.

Functional analysis of the Gro tetramerization domain. (A) Schematic diagram of the p53 tetramerization domain (p53TD)-containing transcription factor chimeras used in this experiment. p53TD is the region of p53 from residue 309 to residue 371. aa, amino acids. (B) The Gro and p53 tetramerization domains are functionally interchangeable for repression. Transient-transfection assays were conducted essentially as described in legend to Fig. 5B with the reporter G₅DE₅tkLuc and expression vectors expressing Dorsal, Twist, and each indicated Gal4 fusion protein. WT, wild type.