Figure 4.

RNA-seq analysis reveals a strong differential gene expression at day 3 of the wound healing process. (A) Principal Component Analysis (PCA) for the four experimental groups at days 3 and 7 of the wound healing process. The first component (PC1) accounts for 20.1%, and the third component (PC3) accounts for 10.1% of the overall variance observed in the data. (B) PCA plot showing the variance between treatment and control groups at day 3. The first two components account for 24.4% and 20.1% of the variance, respectively. (C–E) Volcano plots of differentially expressed genes between treatments (both as monotherapies and in combination) and the control group. Colored dots indicate significantly differentially expressed genes for each experimental group (log₂ fold change > |1| and adjusted p-value < 0.05). (F) Venn diagram showing the number of significantly differentially expressed genes that are either unique for each treatment or overlapping between the three treatments.