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. 2024 Feb 28;14(5):514. doi: 10.3390/diagnostics14050514

Figure 4.

Figure 4

Concordance between Sanger sequencing and NGS. (A) Representative Sanger sequencing chromatogram for a sample with a KRAS_G12D mutation, and (B) the corresponding IGV snapshot of the KRAS mutation detected by NGS. (C) Representative Sanger chromatogram for a sample with a GNAS_R201C mutation, and (D) the corresponding IGV snapshot of the GNAS mutation detected by NGS. (E) A 2 × 2 contingency table shows a comparison of the Sanger and NGS results with 99% agreement between the platforms.