Table 1.

Loss of heterozygosity detection using a panel of 17 STR regions. Listed below are the regions of interest, primer sequences used for amplification, and the expected amplicon size based on the Hg19 genome sequence.

Locus	STR_ Region	Amplicon_ Size	Chr	Start	End	Fwd Primer (5′-3′)	Rev Primer (5′-3′)
1p	D1S1193	180	chr1	12577512	12577692	TCGGCGACATAGCCAGAC	CTTTGATCTAAGGATTACCTAC
1p	D1S407	150	chr1	14854518	14854668	CTGTGCTAACCACATGGAG	GGGATAGAAGGATTAGTAGTG
3p	D3S1539	180	chr3	1064448	1064628	CTCTTTCCATTACTCTCTCC	TTCTCCATCTATCTTTCTCTC
3p	D3S2303	360	chr3	17952254	17952614	TGCCTACATGTTAGTATCCC	CTCCAGAGCTTTGTTTTCAAC
5q	D5S592	150	chr5	119101810	119101960	GGTGTCAACAAAGTAATGTAAAG	TGGATACATATTGTTTTCTGCTG
5q	D5S615	330	chr5	125163290	125163620	GAGATAGGTAGGTAGGTAGG	TCCACAGTGGTAAGAACCAG
9p	D9S251	390	chr9	30819368	30819758	TGCATGTTTTATGTGCACTAAC	CAATACTTTTTAAGGCTTTGTAGG
9p	D9S254	120	chr9	126869098	126869218	TGGGTAATAACTGCCGGAGA	GAGGATAAACCTGCTTCACTCAA
10q	D10S520	180	chr10	96424526	96424706	CAGCCTATGCAACAGAACAAG	GTCCTTGTGAGAAACTGGATGC
10q	D10S523	150	chr10	87006333	87006483	GGTGGAGGTTGTGGTGA	AACTGGGCATTTGTCTTTC
17p	D17S974	180	chr17	10518750	10518930	AGCCTGGGTGAGAGTGAGAC	GCCATTGTTAACAGGTTGGTG
17p	D17S1289	330	chr17	10859282	10859612	GCATGGTCTTTTTCCATTCC	CTGCCTCTAAGCAGTCATTTAGA
17q	D17S1290	240	chr17	56331496	56331736	CAGAGCAAGACTGTCCA	ACCAGGTGTCTCATAAG
18q	D18S51	180	chr18	60948976	60949156	CTCTGAGTGACAAATTGAGACCTTG	ACTTCTCTGGTGTGTGGAGA
18q	D18S1367	330	chr18	64552139	64552469	TTGGTTCATCCAAACATGGTAAA	CGGTGCCCACTAATTTATACCACAC
21q	D21S1244	300	chr21	25269091	25269391	TCTTCTATCTCATATGTGTATC	GGAGGAACTTGAGGATGTG
22q	D22S532	125	chr22	46123159	46123284	CCTGGGCAACAGAGCGAG	GTCTGAGAAGATACTTGATATAG