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. 2024 Feb 24;14(5):488. doi: 10.3390/diagnostics14050488

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Evaluating Qfusion^TM assay performance. (a) Each Qfusion^TM assay was tested with the corresponding cell lines: A549, H2228 (EML4-ALK), LC-2/ad (CCDC6-RET), and HCC78 (SLC45A-ROS1). The A549 was a fusion-negative cell line and Cq was not determined (ND). (b,c) FFPE RNA reference standard was diluted with normal FFPE RNA and analyzed with each Qfusion^TM assay. The x-axis represents the logarithm base 10 value of tumor percentage. Cq values were determined for 15% of tumor samples.