FIG. 5.

Analysis of the association of sorting nexins with membranes. NIH 3T3 cells overexpressing EGF receptors were transiently transfected with epitope-tagged sorting nexin cDNAs. After 26 to 30 h, total-cell extracts (T), and total membrane (M) and (C) cytosolic fractions were prepared as described in Materials and Methods. The distributions of the EGF receptors and the epitope-tagged sorting nexins were determined by Western blotting with an anti-EGF receptor antibody and anti-epitope antibodies, respectively.