FIG. 8.

Differential association of SNX1, SNX1A, SNX2, SNX3, and SNX4 with endogenous transferrin receptors. COS7 cells were transiently transfected with expression vectors encoding epitope-tagged SNX1, SNX1A, SNX2, SNX3, or SNX4. Total-cell extracts were immunoprecipitated (ippt) with an anti-transferrin receptor antibody and electrophoresed, and the resulting blots were probed with either anti-myc or anti-HA antibodies to detect coimmunoprecipitated epitope-tagged sorting nexin molecules. This experiment was repeated three times with similar results.