Figure 1.

Otk and Wnt-4 in embryonic patterning. (A) Loss of segment polarity patterning in a wg^IG22 embryo or denticle lawn phenotype. (B) Loss of segment polarity or naked phenotype due to wg overexpression, as compared to wild-type segment polarity patterning (C). (D) otk-1^RNAi results in denticle pattern disruption but not a clear segment polarity phenotype. (E) otk-2^RNAi showed a disruption of segment polarity, with normally naked cuticles covered in denticles. (F) Co-expression of both otk-1^RNAi and otk-2^RNAi led to a more severe denticle lawn phenotype. The combination of otk-1^RNAi and wnt-4 overexpression (G) led to a slight increase in denticles over otk-1^RNAi alone. The combination of otk-1^RNAi and wnt-4^RNAi showed a loss of cuticle (H), making the observation of patterning phenotypes difficult, but the combination of otk-2^RNAi and wnt-4^RNAi showed a near denticle lawn phenotype (I). For RNAi experiments, we examined embryos n > 100 per experiment.