Figure 5.

Still images from Otk-1, with two fluorescent proteins inserted into the locus, lightsheet imaging in Drosophila embryos. A rapidly folding fluorescent protein (super folder GFP) and a slow folding fluorescent protein (RFP) were inserted directly into the otk-1 locus. (A,A’) RFP signal was only observable in the stomatogastric cells in mid embryonic stages along with a strong sfGFP signal (B,B’) which overlapped (C,C’). In late embryonic stages both sfGFP and RFP were observed in stomatogastric cells (D–F,D’–F’). (G) Quantification of otk1Timer intensity in the ring gland for early and late-stage embryos. Median intensity was rendered via IMARIS. Statistical p-value calculated using unpaired t-test in R. Images and videos are representative of three independent experiments.