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. 2024 Feb 20;25(5):2452. doi: 10.3390/ijms25052452

Figure 4.

Figure 4

Effect of NVs and Extract on cell proliferation. (A–D) Proliferation of HUKE (A,B) and NIH-3T3 (C,D) was assessed by using the MTT colorimetric assay. The effect of different concentrations (30–200 µg/mL) of Extract (A,C) and NVs (B,D) on cell proliferation was assessed at 0, 24 h, and 48 h. Percentage of viability at different time points was calculated relative to the percentage of viability at t.0 and considered 100%. (E–H) Proliferation of HUKE (E,F) and NIH-3T3 (G,H) was assessed by using the MTT colorimetric assay. The effect of conditioned medium (CM) from Extract-treated injured monolayers (E,G) or from NV-treated injured monolayers (F,H) on cell proliferation was assessed at 0, 24 h, and 48 h. Percentage of viability at different time points was calculated relative to the percentage of viability at t.0 and considered 100%. Controls (untreated) are cells treated with unconditioned medium. The results are represented as mean ± SD of three experiments. *** p < 0.0001 vs. untreated cells.