Table 1.
Regulation of epithelial and endothelial junctions by HSPA/Hsp70 chaperones.
| HSPA/Hsp70 Modulation | Experimental System | Effects on Intercellular Junctions | Reference |
|---|---|---|---|
| HSPA1A/Hsp72 siRNA knockdown | Lung (A549) and mammary (MCF-7) epithelial cells | Decreased expression of E-cadherin and occludin | [40] |
| HSPA8/Hsc70 siRNA knockdown | NRK-52E kidney epithelial cells | Decreased expression and junctional accumulation of E-cadherin | [41] |
| HSPA1A/Hsp72 overexpression | TGF-β-stimulated NRK-52E cells | Attenuated TGF-β-induced decrease in E-cadherin expression | [42] |
| Induction of Hsp70s by geranylgeranylacetone (GGA) | TGF-β-stimulated A549 cells | Attenuated TGF-β-induced decrease in E-cadherin expression | [43] |
| HSPA1A/Hsp72 antisense knockdown | Monochloramine-exposed Caco-2 colonic epithelial cells | Promoted oxidant-induced decrease in TEER and an increase in the mannitol flux | [44] |
| HSPA1A/Hsp72 antisense knockdown | C. difficile toxin A-exposed Caco-2 cells | Promoted oxidant-induced decrease in TEER and increase in the mannitol flux | [45] |
| HSPA1A/Hsp72 overexpression | Gliadin-exposed Caco-2 cells | Attenuated gliadin-induced AJ disassembly | [46] |
| HSPA12B overexpression | LPS-treated human umbilical vein endothelial cells (HUVEC) | Attenuated LPS-induced FITC–dextran permeability; restored VE-cadherin expression | [47,48] |
| Induction of Hsp70s by TRC051384 | LPS-treated HUVEC | Attenuated LPS-induced FITC–dextran permeability and restored the decreased E-cadherin, occludin and ZO-1 expression | [49] |
| Combined HSPA1A/Hsp72 and HSPA2/Hsp70-3 knockout in mice | DSS colitis | Promoted DSS-induced gut barrier breakdown and decreased junctional accumulation of ZO-1 | [25] |
| Induction of Hsp70s by GGA in rats | Obstructive nephropathy | Restored E-cadherin expression in the obstructed kidney | [42] |
| Intratracheal administration of HSPA12B siRNA in mice | Cecal ligation and puncture model of sepsis | Increased vascular permeability in septic lungs | [47] |
| Transgenic mice with human HSPA12B overexpression | Myocardial injury and reperfusion | Attenuated vascular leakage; increased ZO-1 expression | [50] |