FIG. 6.

Summary of interactions between PKR and E3 segments in the yeast two-hybrid assay. Strain Y190 was cotransformed with TRP1 plasmids encoding the GAL4 BD alone (vector) or fused with wild-type E3 protein (WT) or the indicated E3 mutant proteins (BD-E3 fusions) and with a LEU2 plasmid encoding the GAL4 AD alone (vector) or fused with the indicated PKR proteins (AD-PKR fusions). Transformants were streaked for single colonies on SC medium containing 30 mM 3-AT and lacking histidine, leucine, and tryptophan. The colony size was scored for each transformant and ranked as −, −/+, +/−, +, 2+, 3+, or 4+. DRBD, dsRNA binding domain; PK, protein kinase domain.