Figure 3.

Iron accumulation affects the adhesion ability of Toxoplasma gondii. (A) The volcano scatter plot shows differential genes under iron accumulation stress through transcriptome analysis. (B) The difference in Toxoplasma adhesion ability in different iron level environments, including transfer to cells after incubating with DFO or Fe²⁺ for 1 h or adding BPDS or Fe²⁺ to the culture medium. As long as the iron ion content increases, the adhesion ability of T. gondii will increase. Means ± SD of three independent experiments, each with three replicates, unpaired two-tailed Student’s t-test, ns: non-significantly.