Figure 11.

AYA22T-aptamers (CTLA4/NKG2A aptamers) enhance NK cell cytolytic activity in vitro. Enriched NK cells isolated from healthy donors’ PBMCs were incubated with recombinant hIL-2 (20 ng/mL) for 24 h. NK cells were then co-cultured with hIFN-$\gamma$-stimulated tumor cells, BT-474 at a ratio of 1:3 (T:E) in the presence or absence of CTLA4/NKG2A aptamers, control aptamer (negative control), and anti-human NKG2A monoclonal antibodies (positive control) for 48 h. Percent cell killing was assessed by lactate dehydrogenase (LDH) assay. The error bars depict the mean ± SD of CD8 T cells from a total of N = 3 healthy donors, each contributing to an independent experimental set. ** p < 0.01. *** p < 0.001.**** p < 0.0001. Ns in the figure devotes non-significant.