Figure 3.

CCG-203971 and CCG-232601 regulate the histone acetylation of H4K12 and H4K16. WI-38 cells were treated with 20 µM of CCG molecules for 24 h. Western blot analysis was used to examine the expression levels of histone H4 acetylation. Total H4 and GAPDH was used as the loading control. (A) Representative Western blots for AcH4K5 and AcH4K8. No change was observed in the expression levels of AcH4K5 and AcH4K8 on the treatment. (B) Representative Western blots for AcH4K12 and AcH4K16. CCG molecules induce the hyperacetylation of H4K12 and H4K16. Blots are representative of three repeats.