Skip to main content
. 2024 Feb 20;25(5):2454. doi: 10.3390/ijms25052454

Figure 2.

Figure 2

Siponimod/BAF312 dose-dependently enhanced neuronal survival in rat primary cultured cortical neurons. Rat cortical neurons were isolated and grown in a 96-well plate with neural basal medium. The neurons were transfected with EGFP, then treated with DMSO (n = 726) or Siponimod/BAF312 at 0.1 nM (n = 585), 0.5 nM (n = 513), or 1.0 nM and imaged. Thousands of individual rat cortical neurons were labelled and tracked. The cumulative hazard curves were plotted, displaying the risk of death for the cortical neurons from one representative experiment. Hazard Ratio (HR) and associated p value were calculated using COX proportional hazards analysis. HR < 1 denotes protection. In the plot, the y-axis represents a quantitative measure of the accumulated risk of cell death over time. Log-rank test was used to determine differences among different concentration of Siponimod/BAF312, with * p < 0.05 considered statistically significant.