Skip to main content
. 2024 Feb 28;25(5):2813. doi: 10.3390/ijms25052813

Figure 4.

Figure 4

Mlkl deficiency exacerbated liver inflammation and had no impact on HFHFrHC diet-induced liver fibrosis. Data from WT, Mlkl−/− or Mlkl+/− mice fed a CD (white box plots) or HFHFrHC diet (box plots). (A) Transcript levels of the inflammatory cytokines TNFα, IL6, IL1β, and Ccl2, normalized to β-microglobulin and represented as the fold change relative to CD fed WT mice (n = 7–8 for WT; 5–8 for Mlkl−/− or Mlkl+/−). (B) Representative PSR staining of liver sections. Scale bar: 50 μM. (C) Quantification of PSR staining, represented as the percentage area (n = 3 per group). (D) Estimation of the total hydroxyproline content (µg/g) in the liver (n = 7–8 for WT; 5–8 for Mlkl−/− or Mlkl+/−). Error bars are represented as mean ± SEM. One-way ANOVA p < 0.05, * p < 0.05, ** p < 0.005, *** p < 0.0005.