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. 2024 Feb 28;25(5):2813. doi: 10.3390/ijms25052813

Figure 5.

Figure 5

Mlkl−/− and Mlkl+/− mice exhibited reduced adiposity in response to HFHFrHC diet feeding. Data from WT, Mlkl−/− or Mlkl+/− mice fed a CD (white box plots) or HFHFrHC diet (box plots). (A) Percentage of epididymal white adipose tissue (eWAT) weight normalized to the body weight (n = 5–8 per/group). (B) H&E-stained sections of eWAT (magnification 20×). (C) Frequency distribution of adipocytes based on their size in eWAT (n = 3/group). (D) Mean surface area of adipocytes in eWAT (n = 3/group); transcript levels of proteins involved adipogenesis (Cebpα, Pparγ) (E), de novo lipogenesis (Fasn, Scd) (F), Pparα (G), and beiging (Ucp2, Cidea, Prdm16, Cox8b), normalized to β-microglobulin (H) (n = 7–8 for WT; 5–8 for Mlkl−/− or Mlkl+/−). Error bars are represented as mean ± SEM. One-way ANOVA p < 0.05, * p < 0.05, ** p < 0.005, *** p < 0.0005.