TABLE 1.
Yeast strains used in this study
| Strain | Description (source) |
|---|---|
| W303-1A | MATa ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 LYS2 (R. Rothstein) |
| W303-1B | MATα ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 (R. Rothstein) |
| YCH199 | W303-1A CDC5-ProA-HIS3-URA3 bar1 |
| YJC1203 | W303-1B spc42::LEU2 TRP1::SPC42-GFP |
| YCH301 | MATa CDC5-proA-HIS3-URA3 bar1 spc42::LEU2 TRP1::SPC42-GFP (segregant of YCH199 × YJC1203) |
| K1983 | MATa cdc4-1 ade2 ura3 his3 (backcrossed 4 times to W303) (K. Nasmyth) |
| YCH212 | MATa cdc4-1 ade2 ura3 his3 CDC5-ProA-HIS3-URA3 (ProA-tagged) CDC5 in K1983) |
| YCH303 | MATaCDC5-ProA-HIS3-URA3 cdc4-1 spc42::LEU2 TRP1::SPC42-GFP, (segregant of YCH212 × YJC1203) |
| K2034-CH-a | MATa cdc13-1 ade2 ura3 his3 (backcrossed 5 times to W303), (K. Nasmyth backcrossed 3 times and then we backcrossed it an additional 3 times to W303) |
| K2034-CH-α | MATα cdc13-1 ade2 ura3 his3 (backcrossed 5 times to W303) (K. Nasmyth backcrossed 3 times and then we backcrossed it an additional 3 times to W303) |
| YCH216 | MATα cdc13-1 ade2 ura3 his3 CDC5-ProA-HIS3-URA3 (ProA-tagged CDC5 in K2034-CH-α) |
| YCH309 | MATa CDC5-ProA-HIS3-URA3 cdc13-1 spc42::LEU2 TRP1::SPC42-GFP (segregant of YCH216 × YJC1203) |
| K2531 | MATa cdc23-1 ade2 leu2 ura3 his3 TRP1 (backcrossed 3 times to W303) (K. Nasmyth) |
| YCH213 | MATa cdc23-1 ade2 leu2 ura3 his3 TRP1 CDC5-ProA-HIS3-URA3 (ProA-tagged CDC5 in K2531) |
| YCH305 | MATa CDC5-ProA-HIS3-URA3 cdc23-1 spc42::LEU2 TRP1::SPC42-GFP (segregant of YCH213 × YJC1203) |
| YJC1413 | W303-1A cdc15-2 ade3 bar1::URA3 (J. Cooper) |
| YCH236 | W303-1A cdc15-2 ade3 bar1::URA3 his3 CDC5-proA-URA3-HIS3 (ProA-tagged CDC5 in YJC191) |
| YCH307 | MATa CDC5-ProA-HIS3-URA3 cdc15-2 spc42::LEU2 TRP1::SPC42-GFP (segregant of YCH236 × YJC1202) |
| YCH208 | W303-1A cdc23-1 bar1::URA3 (segregant from YCH192 × K2531) |
| H5C1A1 | MATa cdc5-1 his7 ural (B. Gavin and L. Hartwell) |
| YCH187 | W303-1A cdc5-1 (H5C1A1 backcrossed 5 times to W303) |
| YCH214 | W303-1A cdc5-1-proA-URA3-LEU2 (ProA-tagged cdc5-1 in YCH187) |
| YCH316 | W303-1A cdc13-1 rad9::URA3 CDC5-ProA-URA3-HIS3 (RAD9 in YCH216 was disrupted with URA3 (pRR330 obtained from P. Burgers was digested with EcoRI and SalI to target RAD9) |
| YOC1523 | W303-1A bar1 PDS1-HA::URA3 mec1-1 (O. Cohen-Fix and D. Koshland) |
| YOC1524 | W303-1A bar1 PDS1-HA::URA3 rad53-21 (O.Cohen-Fix and D. Koshland) |
| YCH317 | W303-1A cdc13-1 mec1-1 CDC5-ProA-HIS3-URA3 (segregant of YCH216 and YOC1523) |
| YCH318 | W303-1A cdc13-1 rad53-21 CDC5-ProA-HIS3-URA3 (segregant of YCH216 and YOC1524) |