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. 2024 Feb 27;25(5):2754. doi: 10.3390/ijms25052754

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Graphical representation of miRNA expression levels in the PC3 prostate cancer cell line analyzed using RT-qPCR, compared to a control group that was not treated. The RNU6 housekeeping gene was used as a reference for normalization and relative expression was calculated using the ΔCT expression/ΔCT control ratio. The cells were irradiated in three doses of 2.5 Gy, with the first dose being 1 × 2.5 Gy, the second dose being 2 × 2.5 Gy, and the third dose being 3 × 2.5 Gy. ** p < 0.01; **** p < 0.0001. N = 2.