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Representative scheme of the workflow for AuNPr-PEG and RT treatment in PC3, DU145, and LNCaP cell lines. Here, the protocol steps are summarized in fundamental sections from plating, treatment with AuNPr-PEG, treatment with radiotherapy (2.5 Gy/fraction), and extraction and analysis of the expression of miRNAs (miRNA-95, miRNA-106-5p, miRNA-145-5p, and miRNA-541-3p).
