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Translation initiation is inhibited in pbp1Δ/pab1Δ strains. Extracts from strains yDM146 (pbp1Δ) (A) and yDM206 (pbp1Δ/pab1Δ) (B) were fractionated on 15 to 50% sucrose gradients, and the optical density at 260 nm (OD260) was monitored. The direction of sedimentation and the positions of the 80S, 60S, and 40S peaks are indicated by arrows.
