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. 2024 Feb 29;25(5):2816. doi: 10.3390/ijms25052816

Figure 5.

Figure 5

Validation of the immune-related lncRNA–mRNA pairs in vitro: (A) Actin-tracker staining results of the control and ISO groups. (B) The mRNA expression levels of Nppa and Nppb in the control and ISO groups. (C,D) The expression levels of lncRNA–mRNA pairs in the control and ISO groups. (E) The expression levels of MIR210HG-regulated immune-related miRNAs in the control and ISO groups. (F) The expression levels of the MIR210HG–BPIFC pair in the siRNA-NC, siRNA-1, and siRNA-2 groups. (G) The expression levels of MIR210HG-regulated immune-related miRNA in the siRNA-NC, siRNA-1, and siRNA-2 groups. (H) The mRNA expression levels of Nppa and Nppb in the siRNA-NC, siRNA-1, and siRNA-2 groups. (I) Actin-tracker staining results of the siRNA-NC, siRNA-1, and siRNA-2 groups. * p-Value < 0.05, ** p-value < 0.01, *** p-value < 0.001, **** p-value < 0.0001, ns: no significance. Control: normal cultured AC16 cells; ISO: ISO-induced AC16 cells; siRNA-NC: NC-FAM-induced AC16 cells; MIR210HG-siRNA-1: one of the siRNAs targeting MIR210HG-induced AC16 cells; MIR210HG-siRNA-2: the other siRNA targeting MIR210HG-induced AC16 cells. Scale bar = 50 µm.