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Extracted ion chromatograms (EICs) and MS1 and MS/MS spectra of N1-acetylspermidine in mouse feces and analytical standard analysis under conventional HILIC-MS and full HILIC-HDX-MS conditions. In HILIC-MS, the EIC at m/z 188.1757, corresponding to [M(H4)+H]+, is displayed. Conversely, in HILIC-HDX-MS, the EIC at m/z 193.2071, corresponding to [M(D4)+D]+, is shown. MS/MS spectra were acquired at stepped normalized collision energies of 20, 30, and 40%.
