Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
A schematic diagram illustrating the development of an enzyme-based biosensor for evaluating the activity of SARS-CoV-2 3CLprotease (3CLpro). (A) The schematic diagram depicts the region of SARS-CoV-2 nonstructural proteins 4 and 5, as well as the amino-terminal region of nsp6, which were cloned into the pcDNA3.1 expression vector with an in-frame V5 epitope tag. (B) The schematic diagram of the pGlo-VRLQS biosensor activated after cleavage by 3CLpro.
