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. 2024 Mar 1;25(5):2871. doi: 10.3390/ijms25052871

Figure 2.

Figure 2

Effects of PHA on the phosphorylation and expression of c-Met, Mcl-1, and Bcl-2 in HSC-3 cells. (A,B) HSC-3 cells were treated with vehicle control (DMSO; 0.1%) or PHA (5 μM) for the indicated times. At each time point, whole-cell lysates were prepared and analyzed by Western blotting to measure the expression levels of phosphorylated (p) and total (T)-c-Met (A) and expression levels of Mcl-1, Bcl-2, and actin (B). (C) HSC-3 cells were treated with vehicle control or PHA (5 μM) in triplicate experiments at 24 h, followed by Western blotting to measure the protein expression levels of Mcl-1, Bcl-2, and actin. (D) The densitometric data in (C) show the protein expression levels of Mcl-1 or Bcl-2 normalized to control actin protein levels. Data are the means ± SE of three independent experiments. * p < 0.05 compared with vehicle control. (E) HSC-3 cells were treated with vehicle control or PHA (5 μM) in triplicate experiments at 24 h. Total cellular RNA was extracted and analyzed by RT-PCR to measure Mcl-1 or β-actin mRNA expression levels. (F) The densitometric data in (E) with the mRNA expression levels of Mcl-1 normalized to control actin mRNA levels. Data are the means ± SE of three independent experiments.