Table 1.

Detection of viable BTV and serotyping of C. sonorensis pools used for plaque assays. Tables indicate which pools were able to generate viable BTV for plaque isolation assays. Additionally, C. sonorensis pools were screened for BTV-10 and BTV-17 via serotype-specific qRT-PCR. Presence of viable virus and serotype detection are indicated by (+). Absence of viable virus and serotype not detected is indicated by (−). (I) indicates an inconclusive serotype result (Ct > 36). Days where C. sonorensis were not available for plaque assay are indicated by (n/a). Additionally, pools of C. sonorensis that did not produce viable virus were not screened for BTV-10 or BTV-17 and are indicated by (−). Finally, plaques from pools that had adequate sequences reads aligning to our BTV-17 reference sequence were not processed for further sequencing and are indicated by (0) in the No. Plaques Sequenced column.

			20 °C				25 °C				30 °C
			PCR Serotype Detection				PCR Serotype Detection				PCR Serotype Detection
dpi	Pool	Plaque Assay	BTV-10	BTV-17	No. Plaques Sequenced	Plaque Assay	BTV-10	BTV-17	No. Plaques Sequenced	Plaque Assay	BTV-10	BTV-17	No. Plaques Sequenced
	A	−	n/a	n/a	n/a	+	I	+	2	+	−	+	2
3	B	−	n/a	n/a	n/a	+	−	+	2	+	−	+	2
	C	−	n/a	n/a	n/a	−	n/a	n/a	n/a	+	−	+	2
	A	+	−	+	2	+	I	+	5	+	+	+	10
7	B	+	−	+	2	+	−	+	2	+	−	+	2
	C	+	−	+	2	+	−	+	2	+	−	+	2
	A	+	−	+	2	+	−	−	2	+	−	+	2
11	B	+	−	−	2	+	−	−	2	+	−	+	0
	C	+	−	+	2	+	−	+	0	+	−	+	0
	A	+	I	+	5	+	I	+	5	+	−	+	0
15	B	+	−	+	2	+	−	+	0	n/a	n/a	n/a	n/a
	C	+	−	+	2	+	−	+	2	n/a	n/a	n/a	n/a
	A	+	−	+	2	n/a	n/a	n/a	n/a	n/a	n/a	n/a	n/a
19	B	+	−	+	0	n/a	n/a	n/a	n/a	n/a	n/a	n/a	n/a
	C	+	+	+	10	n/a	n/a	n/a	n/a	n/a	n/a	n/a	n/a