Skip to main content
. 2024 Feb 22;25(5):2565. doi: 10.3390/ijms25052565

Figure 3.

Figure 3

Characterization of microglia association to amyloid plaques. (A) Representative image of total (IBA1) and human (Ku80) microglia associated with amyloid plaques (Aβ42), and example of surface generation for quantification in Imaris software (version 10.0). Scale bar = 20 μm. Quantification of total (B) or human (C) number of microglial cells in direct contact with the plaque, normalized by the plaque volume. (D) Area covered by microglia in the plaque and surroundings (including a ring of 5 μm from the border of the plaque) in the cortex of 6-month-old hCSF1 5XFAD mice xenotransplanted with WT and ADAD HPCs. Each dot represents one donor line (N = 3 WT + 4 ADAD donor lines, 1–3 xenotransplanted mice per donor line, 3 brain sections analyzed per mouse, 5 plaques quantified per section). (E) In vitro migration assay (scratch wound confluence) in WT, and ADAD iPSC-derived microglia (N = 3 WT + 4 ADAD donor lines, 1–3 independent differentiations per line).