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. 1998 Dec;18(12):7432–7443. doi: 10.1128/mcb.18.12.7432

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Copyright © 1998, American Society for Microbiology

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FIG. 4 — CBFβ-SMMHC sequesters CBFα2 along filaments and aggregates. (A1, A2, and A3) NIH 3T3 cells were cotransfected with pCbfa2 and pCBFB-MYH11. CBFβ-SMMHC localization was detected with antibody β141.2 and a fluorescein-labeled secondary antibody (panel A1), and CBFα2 localization was detected with antibody α3043 and a Texas-red-labeled secondary antibody (panel A2). (B1, B2, and B3) Localization of EGFP-CBFα2 and CBFβ-SMMHC when cotransfected. CBFβ-SMMHC localization is shown by the red signal (β141.2 and a Texas-red-labeled secondary antibody) in panel B1, while EGFP-CBFα2 localization is shown by the green autofluorescent signal in panel B2. (C1, C2, and C3) Cotransfected EGFP-CBFβ-SMMHC (panel C1) and CBFα2 (panel C2), which were detected in a fashion similar to that described for panel B. (A3, B3, and C3) DAPI staining of the respective fields.