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CBFβ-SMMHC colocalizes with actin filaments, while CBFβ does not. NIH 3T3 cells were transfected with pCBFB-MYH11 (A1 and A2), pEGFP-CBFB-MYH11 (B1 and B2), pCBFB (C1 and C2), and pEGFP-CBFB (D1 and D2). Proteins were detected with β141.2 plus a fluorescein isothiocyanate-labeled secondary antibody (panels A1 and C1) and Texas-red-conjugated phalloidin (panels A2, B2, C2, and D2). Panels B1 and D1 show the autofluorescent signal from the GFP fusion proteins.
