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. Author manuscript; available in PMC: 2024 Mar 12.
Published in final edited form as: J Am Soc Mass Spectrom. 2023 Jul 11;34(9):1917–1927. doi: 10.1021/jasms.3c00110

Figure 4.

Figure 4.

UniDec derived Mass plot of semiSWEET in liposomes containing (a) 67%DOPE, 23.2%DOPG, 9.8% 18:1Cardiolipin (CL), (b) 70.9%DOPE, 27.1%DOPG, 2% 16:0–18:1DAG, and (c) 67%DOPE, 23.2%DOPG, 9.8% 18:0Dilysocardiolipin (dilyso-CL). As shown while the semiSWEET is present almost exclusively as a dimer in CL containing liposomes mimicking the gram-negative inner membrane composition, replacing CL with DAG and increasing the curvature leads to almost complete loss of the dimer. In contrast in dilyso-CL containing liposomes semiSWEET is mostly present as a dimer, highlighting the role of CL headgroup chemistry in regulating the dimeric state. The relative percentage of monomer and dimer was obtained from three independent experiment and the number on top of the peaks is mean±s.e. (n=3)