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Establishment of a pancreatic cancer visceral pain model. (A) A schematic depiction of the pancreatic cancer visceral pain protocol. Visceral pain was assessed by an abdominal mechanical hyperalgesia test and the hunch score on days (B,C) 12 (D12), (D,E) 15 (D15), and (F,G) 18 (D18) after the injection of the mPAKPC-luc cells, n=10. A two-sample t-test was used to compare the response frequency, and a Wilcoxon rank-sum test was used to compare the hunch scores. *, P<0.05; **, P<0.01.
